SCSB
X-ray
NEWS
SCSB Crystallography Announcements
July 2011: The Rigaku Ultimate Home Lab and BioSAXS-1000 arrive at UTMB and installation begins. The first data set from the FR-E/R-AXIS-IV gave higher resolution data then from similar crystals at a national synchrotron (name withheld). The only difference is the time span for significant radiation damage goes from hours to a little over a day (24-36 hours). The BioSAXS-1000 is producing excellent SAXS data to exceptionally high q, for a home source.
January 2011: The Center is in the process of purchasing new x-ray diffraction and solution scattering instrumentation for the lab. The new equipment starts with the Rigaku Ultimate Home Lab® which comprises the unsurpassed high-brilliance FR-E++DW Superbright x-ray generator, with the industry standard RAXIS-IV++ crystallography system with both Cu and Cr optics. In addition, we are acquiring the innovative Rigaku BioSAXS-1000, the first SAXS instrument dedicated to biological research in the southern mid-western states, once again marking UTMB as a leader in structural biology resources.
December 2010: XUG Newsletter #3. News about the SAXNS, and CrystalTrak plate codes for intelliplate, Linbro, and CrysChem trays.
May 2010: XUG Newsletter #2. How to keep the Phoenix clean.
October 2009: XUG Newsletter #1. The Hotel arrives.
August 2009: Through a partnership with the Departments of Biochemistry and Molecular Biology , and Neuroscience and Cell Biology, the Sealy Center for Structural Biology and Molecular Biophysics (SCSB) has recently upgraded the X-Ray facilities. The SCSB has purchased the Rigaku CrystalMation Gallery160 (Hotel) for automated crystal tray imaging using our DT-Minstrel. This addition completes the crystallization robotics upgrade began in 2007 with the initial purchase of the Rigaku CrystalMation system.
August 2007: Through a partnership with the Departments of Biochemistry and Molecular Biology , and Neuroscience and Cell Biology, the Sealy Center for Structural Biology and Molecular Biophysics (SCSB) has recently completed an expansion of the X-Ray facilities. In addition to the purchase of XENOCS confocal optics for data collection, SCSB has purchased the Rigaku CrystalMation system to facilitate high-throughput crystallization screening. The platform technology contains three inter-connected robotics systems: The PHOENIX, the Alchemist II, and the Desktop MINSTRAL, which are integrated through the CrystalTrak crystallization database software program. This technology provides SCSB members and collaborators with automation at each step, from custom screen preparation to crystallization trial imaging and analysis. The system was installed in mid August and has seen heavy use since.
January 2007: New SCSB web site launced. The Entire SCSB web site has been revamped. On-line scheduling, for qualified users, is now available for all Instruments.
| PUBLICATIONS | |
| These are Structural publications from the Sealy Center for Structural Biology and Molecular Biophysics X-ray Crystallography Laboratory faculty and staff. | |
| Last Update: May, 2012. | |
2012 | PubMed 2012 |
 |
Bussetta C, Choi KH. Dengue virus nonstructural protein 5 adopts multiple conformations in solution. Biochemistry. 2012 Jul 31;51 (30):5921-31 Epub: Jul 16 {PM} {PDF} |
 |
Zheng J, Gay DC, Demeler B, White MA, Keatinge-Clay AT. Divergence of multimodular polyketide synthases revealed by a didomain structure. Nat Chem Biol 2012 964 Epub: May 27 {PM} {PDF} |
2011 | PubMed 2011 |
 |
Sheng Li, Tamara Tsalkova, Mark A. White, Fang C. Mei, Tong Liu, Daphne Wang, Virgil L. Woods Jr. and Xiaodong Cheng Mechanism of Intracellular cAMP Sensor Epac2 Activation: cAMP-induced conformational changes identified by peptide amide hydrogen/deuterium exchange mass spectrometry (DXMS). J Biol Chem. 2011 286(20);17889-97 Epub: March 17 {PM} {Review} {PDF} |
2010 | PubMed 2010 |
 |
Russo AT, Malmstrom RD, White MA, Watowich SJ. Structural basis for substrate specificity of alphavirus nsP2 proteases. J Mol Graph Model. 2010; 29(1);46-53 Epub]: Apr 24 {PM} {PDF} |
2009 | PubMed 2009 |
 |
Tsalkova T, Blumenthal DK, Mei FC, White MA, Cheng X. Mechanism of EPAC activation: Structural and functional analyses of EPAC2 hinge mutants with constitutive and reduced activities. J Biol Chem. 2009; 284(35);23644-51 Epub: Jun 24 {PM} {PDF} |
 |
Fuson KL, Ma L, Sutton RB, Oberhauser AF, The c2 domains of human synaptotagmin 1 have distinct mechanical properties, Biophys J. 2009; 963);1083-90 EPUB: {PM} {PDF} |
2008 | PubMed 2008 |
 |
Mark Andrew White, Natalia Mast, Ingemar Bjorkhem, Eric F. Johnson, C. David Stout, and Irina A. Pikuleva, The Use of Complementary Cation and Anion heavy-atom salt derivatives to solve the structure of cytochrome P450 46A1, Acta Cryst. D 2008; 65(16);487-95 EPUB: April 20. {PM} {PDF} |
 |
Natalia Mast, Mark Andrew White, Ingemar Bjorkhem, Eric F. Johnson, C. David Stout, and Irina A. Pikuleva, Crystal structures of substrate-bound and substrate-free cytochrome P450 46A1, the principle cholesterol hydroxylase in the brain, PNAS 2008; 105(28);9546-9551 EPUB: July 9. {PM} {PDF} |
2007 | PubMed 2007 |
|
Fuson, K.L., Montes, M., Robert, J. J., Sutton R. B., Structure of Human Synaptotagmin 1 C2A-C2B in the Absence of Ca2+ Reveals a Novel Domain Association, Biochemistry 2008; 46(45);13041-13048 EPUB: Oct. 23. {PM} {PDF} |
 |
Zhao Y, Sun L, Muralidhara BK, Kumar S, White MA, Stout CD, Halpert JR., Structural and Thermodynamic Consequences of 1-(4-Chlorophenyl)imidazole Binding to Cytochrome P450 2B4. Biochemistry 2007 Oct 16;46(41):11559-67. EPUB: Sept 22. {PM} {PDF} |
2006 | PubMed 2006 |
 |
Aditya Hindupur, Deqian Liu, Yonghong Zhao, Henry D. Bellamy, Mark A. White, and Robert O. Fox, The crystal structure of the E. coli stress protein YciF. Protein Science 2006 EPUB: Sept 25. {PM} {PDF} |
 |
Andrew T. Russo, Mark A. White, and Stanley J. Watowich, The Crystal Structure of the Venezuelan Equine Encephalitis Alphavirus nsP2 Protease. Structure 2006 14: 1449-1458. {PM} {PDF} |
 |
Montes M, Fuson KL, Sutton RB, Robert JJ. Purification, crystallization and X-ray diffraction analysis of human synaptotagmin 1 C2A-C2B. Acta Cryst. F 62, Page 926-9, Aug 2006. {PM} {PDF} |
 |
Singh R, White MA, Ramana KV, Petrash JM, Watowich SJ, Bhatnagar A, Srivastava SK., Structure of a glutathione conjugate bound to the active site of aldose reductase. Proteins: Structure, Function, and Bioinformatics 2006, July 1, 64(1), 101-110; [April 25, Epub ahead of print] {PM} {PDF} |
 |
Mukherjee M, Dutta K, White MA, Cowburn D, Fox RO., NMR solution structure and backbone dynamics of domain III of the E protein of tick-borne Langat flavivirus suggests a potential site for molecular recognition. Protein Science 2006, June 15(6), 1342-55. {PM} {PDF} |
 |
Russo AT, Watowich SJ, Purification, crystallization and X-ray diffraction analysis of the C-terminal protease domain of Venezuelan equine encephalitis virus nsP2. Acta Cryst. F 62(6), Page 514-7, June 2006. {PM} {PDF} |
 |
Zhao Y, White MA, Muralidhara BK, Sun L, Halpert JR, Stout CD., Structure of microsomal cytochrome P450 2B4 complexed with the antifungal drug bifonazole: Insight into P450 conformational plasticity and membrane interaction. J Biol Chem. 2006, March 3, 281(9), 5973-81; [Epub ahead of print: Dec 21] {PM} {PDF} |
 |
Sutton RB, Vishnivetskiy SA, Robert J, Hanson SM, Raman D, Knox BE, Kono M, Navarro J, Gurevich VV., Crystal structure of cone arrestin at 2.3A: evolution of receptor specificity., J Mol Biol. 2005 Dec 16;354(5):1069-80. Epub 2005 Nov 2. {PM} {PDF} |
2005 | PubMed 2005 |
 |
Schultz DA, Friedman AM, White MA, Fox RO., The crystal structure of the cis-proline to glycine variant (P114G) of ribonuclease A, Protein Sci. 2005 Nov; 14(11):2862-70. [Epub ahead of print; Sep 30] {PM} {PDF} |
 |
Bi-Hung Peng, Mark A. White, Gerald A. Campbell, Jebamony J. Robert, J. Ching Lee, Roger B. Sutton, Crystallization and preliminary X-ray diffraction of the ZO-binding domain of human occludin Acta Cryst. F 61, Page 369-371, Apr 2005. {PM} {PDF} |
 |
Czerwinski EW, Midoro-Horiuti T, White MA, Brooks EG, Goldblum RM., Crystal structure of Jun a 1, the major cedar pollen allergen from Juniperus ashei, reveals a parallel beta-helical core. J. Biol. Chem 280, 5, 3740-6, 2005. {PM} {PDF} |
2004 | PubMed 2004 |
|
Liu D, Zhao Y, Fan X, Sun Y, Fox RO., Escherichia coli stress protein YciF: expression, crystallization and preliminary crystallographic analysis. Acta Cryst. D 60, Page 2389-90, Dec. 2004. {PM} {PDF} |
 |
Emily E. Scott, Mark A. White, You Ai He, Eric F. Johnson, C. David Stout, and James R. Halpert, Structure of mammalian cytochrome P450 2B4 complexed with 4-(4-chlorophenyl)imidazole at 1.9 A resolution: Insight into the range of P450 conformations and coordination of redox partner binding J. Biol. Chem 279, 26, 27294-301, 2004. {PM} {PDF} |
|
Liu D, Zhao Y, Fan X, Sun Y, Fox RO, Expression, crystallization and preliminary crystallographic analysis of YciE, a stress protein from Escherichia coli. Acta Cryst. D 60, Page 1888-9, Oct. 2004. {PM} {PDF} |
2003 | PubMed 2003 |
 |
Yonghong Zhao, Deqian Liu, Warna D. Kaluarachi, Henry D. Beleamy, Mark A. White, and Robert O. Fox, (2003), The crystal structure of Escherichia coli heat shock protein YedU reveals three potential catalytic active sites, Protein Science 12, 2303-11, 2003. {PM} {PDF} |
 |
Emily E. Scott, You Ai He, Michael R. Webster, Mark A. White, Christopher C. Chin, James R. Halpert, Erik F. Johnson, and C. David Stout, (2003), An open conformation of mammalian cytochrome P450 2B4 at 1.6-A resolution, PNAS 100, 13196-13201, 2003. {PM} {PDF} |
 |
Mark A. White, Deqian Liu, Michael R. Holbrook, Robert E. Shope, Alan D. T. Barrett and Robert O. Fox, (2003), Crystallization and preliminary X-ray diffraction analysis of Langat virus envelope protein domain III, Acta Cryst. D 59, 1049-51, 2003. {IUCr} {PDF} |
 |
Deqian Liu, Terumi Midoro-Horiuti, Mark A. White, Edward G. Brooks, Randall M. Goldblum and Edmund W. Czerwinski (2003), Crystallization and preliminary X-ray diffraction analysis of Jun a 1, the major allergen isolated from pollen of the mountain cedar Juniperus ashei, Acta Cryst. D 59, 1052-53, 2003. {IUCr} {PDF} |
 |
Landau EM, Pebay-Peyroula E, Neutze R. Structural and mechanistic insight from high resolution structures of archaeal rhodopsins. FEBS Lett. 555(1), Page 51-6. Nov 27, 2003. {PM} {PDF} |
2002 | PubMed 2002 |
2001 | PubMed 2001 |
 |
John O. Wooll, Robert H. E. Friesen, Mark A. White, Stanley J. Watowich, Robert O. Fox, J. Ching Lee* and Edmund W. Czerwinski*, (2001), Structural and Functional Linkages Between SubunitInterfaces in Mammalian Pyruvate Kinase, J. Mol. Biol. 312, 525-540, 2001. {PM} {PDF} |
2000 | PubMed 2000 |
1999 | PubMed 1999 |
 |
Mark A. White, Stanley J. Watowich, and Robert O. Fox, (1999), Calibration of Spiral Readout Image Plate Detectors, J. Appl. Cryst. 32, 65-70, 1999. {IUCr} {PDF} |
Calendar of Crystallographic Meetings
| Jan. 5-7, 2011 | CCP4 Study Weekend: Warwick, UK. (info) |
| Jan 31- Feb. 2, 2011 | Winter school of Synchrotron Radiation: Liptovsky Jan, Slovakia. (info) |
| March 5-9th, 2011 | Biophysical Society: Baltimore, MD. (info) |
| March 25-27, 2011 | Texas Protein Folders: Camp Allen, TX. (info) |
| April 8th, 2011 | SCSB Structural Biology Symposium: , Galveston, TX (info) |
| April 11-14, 2011 | The British Crystallographic Association spring meeting:Keele, UK. (info) |
| April 3-4, 2011 | RapiData Practical Course: BNL, Long Island ,NY (info) |
| April 28- May 3, 2011 | EMBO Practical Course: "Structural and biophysical methods for biological macromolecules in solution", Grenoble, (info) |
| May 10-14, 2011 | INTERNATIONAL CONFERENCE ON STRUCTURAL GENOMICS: Toronto, CA. (info) |
| May 16-20, 2011 | EMBO Practical Course: "Small angle neutron and X-ray scattering from proteins in solution", Grenoble, (info) |
| May 28- June 2, 2011 | ACA: New Orleans, LAl. (info) |
| June 2-12, 2011 | 43rd. Int. School od Crystallography: Erice, Italy (info) |
| June 15-18, 2010 | The X6A workbench: Advanced Structural Biology Tools: Brookhaven, NY. (info) |
| June 7-15, 2011 | CCP4/APS Summer School: Chicago. (info) |
| June 30- July 1, 2011 | Advances in Protein Crystallography: Hamburg, DL. (info) |
| July 4-13, 2011 | EMBO Practical Course: "High throughput methods for protein production and crystallization", Grenoble, (info) |
| July 15-20, 2012 | Gordon Conference on Diffraction Methods: Lewiston, MN. (info) |
| July 23-27, 2011 | Protein Society: Boston. MA (info) |
| Sept. 4-7, 2011 | ECMS 2011: European Crystallography Meeting: Potsdam, DL. (info) |
| Sept. ?, 2011 | The British Crystallographic Association BACG Meeting:Manchester, UK. (info) |
| Sept. 12, 2011 | 9th International NCCR Symposium on New Trends in Structural Biolog: ETH Zurich, Switzerland. (info) |
| Oct. ?, 2011 | 19th Annual SouthWest Macromolecular Symposium: Woodlands, TX. (info) |